Glen or Glenda

Scientist

Bela Lugosi

Glen / Glenda (as Daniel Davis)

Edward D. Wood Jr.

Inspector Warren

Lyle Talbot

Dr. Alton / Narrator

Timothy Farrell

Barbara

Dolores Fuller

Alan / Anne

'Tommy' Haynes

Johnny (as Charles Crafts)

Charlie Crafts

Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Conrad Brooks

Man with Hat and Receding Hairline (uncredited)

Henry Bederski

Woman in Nightmare (uncredited)

Carol Daugherty

The Devil / Glen's Father (uncredited)

'Captain' DeZita

(uncredited)

Helen Miles

Miss Stevens (uncredited)

Shirley Speril

Minor Role (uncredited)

Amzie Strickland

Man in Nightmare (uncredited)

Harry Thomas

Judge (uncredited)

William C. Thompson

Patrick / Patricia (uncredited)

'Mr. Walter' Hadjwiecyz

Man at Transvestite's Suicide (uncredited)

George Weiss

Sheila, Glen's Sister (uncredited)

Evelyn Wood